Last updated: 2021-12-17

Checks: 2 0

Knit directory: sct2_revision/

This reproducible R Markdown analysis was created with workflowr (version 1.6.2). The Checks tab describes the reproducibility checks that were applied when the results were created. The Past versions tab lists the development history.


Great! Since the R Markdown file has been committed to the Git repository, you know the exact version of the code that produced these results.

Great! You are using Git for version control. Tracking code development and connecting the code version to the results is critical for reproducibility.

The results in this page were generated with repository version 0848de4. See the Past versions tab to see a history of the changes made to the R Markdown and HTML files.

Note that you need to be careful to ensure that all relevant files for the analysis have been committed to Git prior to generating the results (you can use wflow_publish or wflow_git_commit). workflowr only checks the R Markdown file, but you know if there are other scripts or data files that it depends on. Below is the status of the Git repository when the results were generated:


Ignored files:
    Ignored:    .Rhistory
    Ignored:    .Rproj.user/
    Ignored:    data/raw_data/
    Ignored:    data/rds_filtered/
    Ignored:    data/rds_raw/
    Ignored:    data/sampled_counts/
    Ignored:    output/snakemake_output/

Untracked files:
    Untracked:  code/02_run_seurat_noclip.R
    Untracked:  code/07A_muscat_simulate_deseq2.R
    Untracked:  code/07A_muscat_simulate_mast_wilcox.R
    Untracked:  code/07A_simulate_muscat.R
    Untracked:  code/07B_muscat_process.R
    Untracked:  code/07B_muscat_process_deseq2.R
    Untracked:  code/07B_muscat_process_mast_wilcox.R
    Untracked:  code/07B_process_muscat.R
    Untracked:  code/08_run_presto.R
    Untracked:  code/17A_HEK_SS3_dropseq.Rmd
    Untracked:  code/17A_HEK_SS3_dropseq_files/
    Untracked:  code/17C_HEK_Quartzeseq2_dropseq.Rmd
    Untracked:  code/17C_HEK_Quartzeseq2_dropseq_files/
    Untracked:  code/17_HEK_SS3_ChromiumV3.Rmd
    Untracked:  code/17_HEK_SS3_ChromiumV3.nb.html
    Untracked:  code/17_HEK_SS3_ChromiumV3_files/
    Untracked:  code/AA_process_muscat.R
    Untracked:  code/BB_process_muscat.R
    Untracked:  code/DD_simulate_muscat.R
    Untracked:  code/EE_simulate_muscat.R
    Untracked:  code/XX_process_muscat.R
    Untracked:  code/XX_simulate_muscat.R
    Untracked:  code/YY_simulate_muscat.R
    Untracked:  code/ZZ_simulate_muscat.R
    Untracked:  code/kang_muscat.R
    Untracked:  code/prep_sce.R
    Untracked:  code/prep_sce_ss3_dropseq.R
    Untracked:  data/azimuth_predictions/
    Untracked:  junk/
    Untracked:  mamba_update_changes.txt
    Untracked:  output/11C_VST/
    Untracked:  output/AAmuscat_simulated/
    Untracked:  output/BBmuscat_simulated/
    Untracked:  output/CCmuscat_simulated/
    Untracked:  output/CD4_NK_downsampling_DE.rds
    Untracked:  output/DDmuscat_simulated/
    Untracked:  output/EEmuscat_simulated/
    Untracked:  output/KANGmuscat_simulated/
    Untracked:  output/NK_downsampling/
    Untracked:  output/XXmuscat_simulated/
    Untracked:  output/YYmuscat_simulated/
    Untracked:  output/ZZmuscat_simulated/
    Untracked:  output/figures/
    Untracked:  output/kang_prepsce.rds
    Untracked:  output/muscat_simulated/
    Untracked:  output/muscat_simulation/
    Untracked:  output/seu_sct2_sim.rds
    Untracked:  output/simulation_HEK_QuartzSeq2_Dropseq_downsampling/
    Untracked:  output/simulation_HEK_SS3_ChromiumV3_downsampling/
    Untracked:  output/simulation_HEK_SS3_Dropseq_downsampling/
    Untracked:  output/simulation_HEK_downsampling/
    Untracked:  output/simulation_NK_downsampling/
    Untracked:  output/ss3_dropseq_prepsim.rds
    Untracked:  output/tables/
    Untracked:  output/vargenes/
    Untracked:  snakemake/.snakemake/
    Untracked:  snakemake/Snakefile_noclip.smk
    Untracked:  snakemake/Snakefile_presto.smk
    Untracked:  snakemake/cluster.yaml
    Untracked:  snakemake/install_glm.R
    Untracked:  snakemake/jobscript.sh
    Untracked:  snakemake/jobscript_ncells.sh
    Untracked:  snakemake/local_run_downsampling.sh
    Untracked:  snakemake/local_run_glm.sh
    Untracked:  snakemake/local_run_ncells.sh
    Untracked:  snakemake/local_run_noclip.sh
    Untracked:  snakemake/local_run_presto.sh
    Untracked:  snakemake/local_run_time.sh
    Untracked:  snakemake/run_glm.sh
    Untracked:  snakemake/run_ncells.sh
    Untracked:  snakemake/sct2_revision_env.yml
    Untracked:  temp_figures/

Unstaged changes:
    Deleted:    analysis/04_PBMC68k.Rmd
    Modified:   code/02_run_seurat.R
    Modified:   code/03_run_vst2_downsample.R
    Modified:   code/04_run_vst_ncells.R
    Modified:   code/06_run_sct.R
    Modified:   data/datasets.csv
    Modified:   snakemake/Snakefile_downsampling.smk
    Modified:   snakemake/Snakefile_glm_seurat.smk
    Modified:   snakemake/Snakefile_metacell.smk

Note that any generated files, e.g. HTML, png, CSS, etc., are not included in this status report because it is ok for generated content to have uncommitted changes.


These are the previous versions of the repository in which changes were made to the R Markdown (analysis/index.Rmd) and HTML (docs/index.html) files. If you’ve configured a remote Git repository (see ?wflow_git_remote), click on the hyperlinks in the table below to view the files as they were in that past version.

File Version Author Date Message
Rmd 0848de4 Saket Choudhary 2021-12-17 workflowr::wflow_publish(“analysis/index.Rmd”)
html 0a2e80d Saket Choudhary 2021-12-17 Build site.
Rmd 8afc486 Saket Choudhary 2021-12-17 workflowr::wflow_publish("analysis/*")
html d736ec8 Saket Choudhary 2021-07-07 Build site.
html 400797a Saket Choudhary 2021-07-06 workflowr::wflow_git_commit(all = TRUE)
Rmd ccb0fb4 Saket Choudhary 2021-07-06 workflowr::wflow_git_commit(all = TRUE)
Rmd e0b7c2c Saket Choudhary 2021-07-06 Start workflowr project.

Create Datasets

Run QC

Run DE Simulation

Figures